6FDC

Crystal structure of the PDE4D catalytic domain in complex with GEBR-32a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-05
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	64.692, 98.608, 120.165
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.324 - 1.450
R-factor	0.1792
Rwork	0.178
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.038
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.324	1.763
High resolution limit [Å]	1.450	1.450
Rmerge	0.060
Number of reflections	263295
<I/σ(I)>	21.5
Completeness [%]	100.0
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Hepes PEG 3350 Magnesium Chloride