6FD0
Human KIBRA C2 domain mutant M734I S735A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-03 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.240, 49.049, 49.549 |
| Unit cell angles | 62.71, 72.07, 83.71 |
Refinement procedure
| Resolution | 26.556 - 2.642 |
| R-factor | 0.210527740854 |
| Rwork | 0.207 |
| R-free | 0.29446 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fb4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.067 | 0.565 |
| Number of reflections | 7811 | 333 |
| <I/σ(I)> | 18.7 | 1.8 |
| Completeness [%] | 98.3 | 86.5 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.15M ammonium sulfate, 0.1M MES pH 6.0, 15% w/v PEG4000 |
