6FCP
Crystal structure of 14-3-3 sigma in complex with Shroom3 P1244L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.533, 112.185, 62.529 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.548 - 1.450 |
R-factor | 0.132 |
Rwork | 0.131 |
R-free | 0.15840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lw1 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.885 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.550 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmeas | 0.074 | 0.712 |
Number of reflections | 51510 | 2541 |
<I/σ(I)> | 21.1 | 4.1 |
Completeness [%] | 99.6 | |
Redundancy | 12.9 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.095 M HEPES pH7.3 0.19M CaCl2 26% v/v PEG400 5% v/v glycerol |