6FC7
Crystal Structure of Two-Domain Laccase mutant H165F from Streptomyces griseoflavus with high copper ions occupancy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.27516 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.025, 94.585, 115.931 |
| Unit cell angles | 90.00, 90.00, 92.01 |
Refinement procedure
| Resolution | 49.415 - 1.950 |
| R-factor | 0.2121 |
| Rwork | 0.195 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mkm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.995 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Number of reflections | 235591 | 41396 |
| <I/σ(I)> | 7.88 | 1.57 |
| Completeness [%] | 94.3 | 92.9 |
| Redundancy | 3.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG 6K, 0.1M Bicine, pH 9 |
