6FC0
Crystal structure of the eIF4E-eIF4G complex from Chaetomium thermophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.587, 75.169, 48.427 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.710 - 1.293 |
R-factor | 0.1654 |
Rwork | 0.163 |
R-free | 0.20090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fbz chain A |
RMSD bond length | 0.008 |
RMSD bond angle | 0.941 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.710 | 1.360 |
High resolution limit [Å] | 1.290 | 1.290 |
Number of reflections | 54870 | 7178 |
<I/σ(I)> | 11.5 | 1.6 |
Completeness [%] | 93.9 | 85.3 |
Redundancy | 3.1 | 3 |
CC(1/2) | 0.584 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10% w/v PEG 20000 20% PEG 500 MME 0.03 M diethyleneglycol 0.03 M triethyleneglycol 0.03 M tetraethyleneglycol 0.03 M pentaethyleneglycol 0.1 M Bicine/Trizma pH 8.5 |