6FBZ

Crystal structure of the eIF4E-eIF4G complex from Chaetomium thermophilum in the cap-bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	63.960, 75.354, 48.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.763 - 1.496
R-factor	0.1718
Rwork	0.170
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tpw
RMSD bond length	0.022
RMSD bond angle	1.669
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.350	1.520
High resolution limit [Å]	1.500	1.500
Number of reflections	38441	1742
<I/σ(I)>	16	1.2
Completeness [%]	99.6	91.6
Redundancy	12.7	10.7
CC(1/2)		0.416

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10% w/v PEG 20000 20% PEG 500 MME 0.02 M 1,6-hexanediol 0.02 M 1-butanol 0.02 M (RS)-1,2-propanediol 0.02 M 2-propanol 0.02 M 1,4-butanediol 0.02 M 1,3-propanediol 0.1 M Bicine/Trizma pH 8.5 0.687 mM m7GpppG