6FB2
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.89 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.889, 67.362, 90.867 |
Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
Resolution | 41.986 - 2.950 |
R-factor | 0.1916 |
Rwork | 0.187 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g9z |
RMSD bond length | 0.003 |
RMSD bond angle | 0.803 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.570 | 3.110 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.080 | 0.500 |
Number of reflections | 11150 | |
<I/σ(I)> | 9.4 | |
Completeness [%] | 97.9 | |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M calcium acetate, 0.1 M sodium acetate pH 4.6-5.4, 33-40% (v/v) 1,2-propanediol |