6FAD
SR protein kinase 1 (SRPK1) in complex with the RGG-box of HSV1 ICP27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9282 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 99.980, 99.980, 426.049 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 80.210 - 2.801 |
R-factor | 0.2009 |
Rwork | 0.198 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wak |
RMSD bond length | 0.008 |
RMSD bond angle | 0.966 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.210 | 2.901 |
High resolution limit [Å] | 2.801 | 2.801 |
Rmerge | 0.095 | 0.753 |
Rmeas | 0.100 | 0.800 |
Rpim | 0.031 | 0.268 |
Number of reflections | 60695 | 5993 |
<I/σ(I)> | 17.5 | 2.54 |
Completeness [%] | 100.0 | 99.97 |
Redundancy | 10.1 | 8.9 |
CC(1/2) | 0.994 | 0.812 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.09 M (Sodium nitrate, 0.3 Sodium phosphate, 0.3 M ammonium sulphate), 0.1 M (Sodium HEPES, MOPS) buffer system, 50% v/v GOL_P4K mix [Morpheus HT96 C7, Molecular Dimensions] |