6F8W
Crystal structure of the PDE4D catalytic domain in complex with GEBR-18a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.039, 98.717, 120.765 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.690 - 1.601 |
| R-factor | 0.1927 |
| Rwork | 0.191 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6f6u |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.928 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.690 | 1.889 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | |
| Number of reflections | 196940 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Hepes PEG 3350 Magnesium Chloride |
