6F8H
antitoxin GraA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2014-10-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.740, 48.950, 66.930 |
| Unit cell angles | 90.00, 92.62, 90.00 |
Refinement procedure
| Resolution | 46.806 - 2.002 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.124 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.810 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.340 |
| Number of reflections | 27427 | 2703 |
| <I/σ(I)> | 19.67 | 3.71 |
| Completeness [%] | 99.3 | 98.11 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.970 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M KCl, 0.1 M HEPES pH 7.5, 15%(w/v) PEG 6000 |
