6F7I
human MALT1(329-728) IN COMPLEX WITH MLT-747
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-29 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.200, 108.970, 73.510 |
| Unit cell angles | 90.00, 111.79, 90.00 |
Refinement procedure
| Resolution | 68.260 - 2.430 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1XEJI |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.260 | 2.430 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.045 | 0.588 |
| Number of reflections | 35514 | |
| <I/σ(I)> | 16.6 | 2.1 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 12 mg/ml, 25mM HEPES pH7.5, 50mM NaCl, 1mM TCEP |
