6F7H
Crystal structure of human AQP10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2016-06-10 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.071, 116.844, 138.549 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.535 - 2.304 |
| R-factor | 0.1903 |
| Rwork | 0.189 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.453 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASES |
| Refinement software | PHENIX ((1.12rc2_2821: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.119 | |
| Number of reflections | 69920 | |
| <I/σ(I)> | 12.5 | 1.6 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 7.84 | 7.62 |
| CC(1/2) | 0.990 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 100 mM MES-monohydrate-NaOH pH 6.0 19% PEG 2k MME 5% glycerol |
