6F7A
Gloeobacter Ligand-gated Ion Channel (GLIC) closed state crystallized in an ultra-swollen lipidic mesophase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.940, 208.220, 255.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.201 - 6.000 |
| R-factor | 0.2878 |
| Rwork | 0.286 |
| R-free | 0.31840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4npq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.201 | 6.215 |
| High resolution limit [Å] | 6.000 | 6.000 |
| Rmeas | 0.420 | |
| Number of reflections | 28508 | 533 |
| <I/σ(I)> | 4.99 | |
| Completeness [%] | 98.0 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.980 | 0.130 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 298 | 0.2 M (NH4)SO4, 0.02M NaCl, 0.02M Na Act 4 pH, 33%v/v PEG200 |
