6F6W
Structure of Mycobacterium smegmatis RNA polymerase core
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.81 |
Software | RELION,Coot,REFMAC |
Symmetry | C1 |
CTF correction | PHASE FLIPPING ONLY |