6F5Q
Crystal Structure of KDM4D with GF026 ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.437, 72.437, 151.666 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.528 - 1.430 |
R-factor | 0.1438 |
Rwork | 0.143 |
R-free | 0.17360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ets |
RMSD bond length | 0.010 |
RMSD bond angle | 1.138 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.528 | 48.528 | 1.510 |
High resolution limit [Å] | 1.430 | 4.260 | 1.430 |
Rmerge | 0.090 | 0.030 | 1.800 |
Rmeas | 0.099 | 0.033 | 1.978 |
Number of reflections | 143054 | 5336 | 22737 |
<I/σ(I)> | 13.14 | 50.76 | 0.88 |
Completeness [%] | 99.6 | 99.6 | 97.8 |
Redundancy | 5.754 | 5.932 | 5.7 |
CC(1/2) | 0.999 | 0.999 | 0.395 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 100mM HEPES, 180mM ammonium sulphate, 24% PEG 3350 |