6F5F
Structure of ARTD2/PARP2 WGR domain bound to double strand DNA with 5 nucleotide overhang and 5'phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.150, 87.150, 185.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 2.980 |
R-factor | 0.23056 |
Rwork | 0.228 |
R-free | 0.27530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 6F1K |
RMSD bond length | 0.011 |
RMSD bond angle | 1.778 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.000 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.069 | |
Rmeas | 0.075 | |
Number of reflections | 23275 | |
<I/σ(I)> | 15.29 | |
Completeness [%] | 99.8 | |
Redundancy | 6.38 | |
CC(1/2) | 0.880 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Na-formate 20 % PEG 3350 20 % glycerol |