6F4P

Human JMJD5 in complex with MN, NOG and RPS6 (129-144) (complex-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.283, 64.925, 78.037
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.460 - 1.450
R-factor	0.1473
Rwork	0.146
R-free	0.16820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f4o
RMSD bond length	0.009
RMSD bond angle	1.005
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.460	1.500
High resolution limit [Å]	1.450	1.450
Rmerge	0.090	1.200
Rpim	0.035	0.497
Number of reflections	44727	4347
<I/σ(I)>	19	1.8
Completeness [%]	98.8	97.7
Redundancy	8.8	8.4
CC(1/2)	0.997	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.1M Bis-Tris pH 6.0, 26.4 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:2 ratio)