6F4I

Crystal structure of Drosophila melanogaster SNF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918417
Spacegroup name	P 21 21 21
Unit cell lengths	52.309, 119.653, 122.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.329 - 1.490
R-factor	0.1485
Rwork	0.146
R-free	0.18820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1urn
RMSD bond length	0.016
RMSD bond angle	1.521
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.490	1.490
Rmeas	0.034
Number of reflections	125117
<I/σ(I)>	22.15
Completeness [%]	98.6
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	0.1 M Tris-HCl, pH 7.5, 0.2 M sodium chloride, 1 M sodium citrate