6F1H
C1rC1s complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.209, 88.340, 197.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.645 - 4.500 |
| R-factor | 0.3052 |
| Rwork | 0.303 |
| R-free | 0.33980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lor |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.734 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2722) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.300 | 4.660 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.164 | |
| Number of reflections | 9382 | |
| <I/σ(I)> | 5.4 | 1.9 |
| Completeness [%] | 98.1 | 98.5 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 12-18% PEG 8000, 100 mM Imidazole at pH 8.0 |
