6F08
14-3-3 zeta in complex with the human Son of sevenless homolog 1 (SOS1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 193.15 |
Detector technology | PIXEL |
Collection date | 2017-05-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 2.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.059, 93.140, 74.519 |
Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
Resolution | 74.420 - 1.900 |
R-factor | 0.21282 |
Rwork | 0.210 |
R-free | 0.26732 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qjb |
RMSD bond length | 0.017 |
RMSD bond angle | 1.775 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.420 | 1.949 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.112 | |
Rmeas | 0.135 | |
Rpim | 0.075 | |
Number of reflections | 68382 | |
<I/σ(I)> | 2.34 | |
Completeness [%] | 99.9 | |
Redundancy | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.1 M phosphate citrate pH 4.2, 36% (v/v) PEG 300 |