6F02
Crystal structure of human glycosylated kallistatin at 3.0 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-06 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 138.020, 138.020, 157.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 104.150 - 3.000 |
| R-factor | 0.2357 |
| Rwork | 0.234 |
| R-free | 0.27551 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qlp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.157 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 104.150 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.963 | |
| Rpim | 0.548 | |
| Number of reflections | 29350 | 4755 |
| <I/σ(I)> | 9.4 | 1.3 |
| Completeness [%] | 94.6 | 96.7 |
| Redundancy | 5.7 | 5.6 |
| CC(1/2) | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | PEG3350, Salt |
