6EZE

The open conformation of E.coli Elongation Factor Tu in complex with GDPNP.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-01-26
Detector	MAR CCD 130 mm
Wavelength(s)	1.0379
Spacegroup name	P 21 21 2
Unit cell lengths	61.243, 243.589, 67.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.697 - 2.470
R-factor	0.1742
Rwork	0.172
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1efc
RMSD bond length	0.002
RMSD bond angle	0.590
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_2614: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.700	2.560
High resolution limit [Å]	2.470	2.470
Rmerge	0.104	0.763
Number of reflections	36491	3509
<I/σ(I)>	18.1	4.2
Completeness [%]	98.3	95.64
Redundancy	7.8	7
CC(1/2)	0.996	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Protein konc. 8 mg/ml in 30 mM KCl, 20mM Hepes pH 7.5,5mM MgCl2, 1mM DTT 1 ul protein sample mixed with 1ul reservoir. Reservoir: 2M Ammonium sulfate 100 mM Hepes pH 7.5 2% (v/v) PEG 400 1mM DTT and streak seeded.