6EY6
C-terminal part (residues 315-516) of PorM with the llama nanobody nb130
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-21 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 |
Unit cell lengths | 55.245, 77.179, 156.302 |
Unit cell angles | 90.24, 91.76, 97.16 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.210 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.065 | 0.764 |
Rpim | 0.433 | |
Number of reflections | 145898 | 221179 |
<I/σ(I)> | 12.5 | 2 |
Completeness [%] | 97.6 | 96.8 |
Redundancy | 2.9 | 2.9 |
CC(1/2) | 0.647 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M ammonium citrate tribasic pH 7.0 20%(w/v) PEG3350 |