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5FWO

Llama nanobody PorM_130

Summary for 5FWO
Entry DOI10.2210/pdb5fwo/pdb
DescriptorNANOBODY (2 entities in total)
Functional Keywordsimmune system, type-9 secretion system (t9ss), nanobody
Biological sourceLAMA GLAMA (LLAMA)
Total number of polymer chains1
Total formula weight14994.61
Authors
Leone, P.,Roussel, A. (deposition date: 2016-02-18, release date: 2017-05-03, Last modification date: 2024-10-09)
Primary citationDuhoo, Y.,Roche, J.,Trinh, T.T.N.,Desmyter, A.,Gaubert, A.,Kellenberger, C.,Cambillau, C.,Roussel, A.,Leone, P.
Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.
Acta Crystallogr F Struct Biol Commun, 73:286-293, 2017
Cited by
PubMed Abstract: PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM.
PubMed: 28471361
DOI: 10.1107/S2053230X17005969
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.7 Å)
Structure validation

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