5FWO
Llama nanobody PorM_130
Summary for 5FWO
Entry DOI | 10.2210/pdb5fwo/pdb |
Descriptor | NANOBODY (2 entities in total) |
Functional Keywords | immune system, type-9 secretion system (t9ss), nanobody |
Biological source | LAMA GLAMA (LLAMA) |
Total number of polymer chains | 1 |
Total formula weight | 14994.61 |
Authors | Leone, P.,Roussel, A. (deposition date: 2016-02-18, release date: 2017-05-03, Last modification date: 2024-10-09) |
Primary citation | Duhoo, Y.,Roche, J.,Trinh, T.T.N.,Desmyter, A.,Gaubert, A.,Kellenberger, C.,Cambillau, C.,Roussel, A.,Leone, P. Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis. Acta Crystallogr F Struct Biol Commun, 73:286-293, 2017 Cited by PubMed Abstract: PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM. PubMed: 28471361DOI: 10.1107/S2053230X17005969 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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