6EW9

CRYSTAL STRUCTURE OF DEGS STRESS SENSOR PROTEASE IN COMPLEX WITH ACTIVATING DNRLGLVYQF PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	P 1 2 1
Unit cell lengths	71.600, 53.650, 132.500
Unit cell angles	90.00, 101.80, 90.00

Refinement procedure

Resolution	43.382 - 2.200
R-factor	0.228
Rwork	0.226
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	129.699		2.260
High resolution limit [Å]	2.200	9.840	2.200
Rmerge	0.162	0.033	1.493
Rmeas	0.175	0.037	1.620
Number of reflections	50358	612	3680
<I/σ(I)>	8.13	38.66	0.94
Completeness [%]	99.7	97.9	99.6
Redundancy	6.72		6.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	14.4% PEG 8000, 160 mM CaAc, 80 mM Na-cacodylate pH 6.5, 20% glycerol