6EW8
Crystal structure of the BCL6 BTB domain in complex with anilinopyrimidine ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 30.668, 73.017, 53.995 |
Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
Resolution | 36.510 - 1.840 |
R-factor | 0.185 |
Rwork | 0.182 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.6) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.510 | 1.940 |
High resolution limit [Å] | 1.840 | 1.840 |
Number of reflections | 8260 | |
<I/σ(I)> | 18.9 | 4.4 |
Completeness [%] | 82.9 | 34.4 |
Redundancy | 3.3 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 310 | unknown |