6EUR
Crystal structure of the complex Fe(II)/alpha-ketoglutarate dependent dioxygenase KDO5 with Fe(II)/alpha-ketoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97855 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.420, 99.460, 166.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.670 - 2.300 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.067 | 1.480 |
| Number of reflections | 59249 | |
| <I/σ(I)> | 0.159 | 1.12 |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Imidazole malate pH 7.0 25% w/v PEG 4000 0.15M Li2SO4 |
