6ES8
HIV capsid hexamer with IP6 ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 277 |
Detector technology | CCD |
Collection date | 2017-01-03 |
Detector | OXFORD RUBY CCD |
Wavelength(s) | 1 |
Spacegroup name | P 6 |
Unit cell lengths | 91.299, 91.299, 57.201 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.390 - 1.900 |
R-factor | 0.18763 |
Rwork | 0.186 |
R-free | 0.22372 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hgp |
RMSD bond length | 0.013 |
RMSD bond angle | 1.931 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.084 | 0.878 |
Number of reflections | 21569 | |
<I/σ(I)> | 10.8 | 1.6 |
Completeness [%] | 91.5 | 94.1 |
Redundancy | 4.6 | 4.4 |
CC(1/2) | 0.943 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 277 | PEG4K |