6ERK
Crystal structure of diaminopelargonic acid aminotransferase from Psychrobacter cryohalolentis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.069, 67.910, 118.070 |
| Unit cell angles | 90.00, 128.81, 90.00 |
Refinement procedure
| Resolution | 70.940 - 1.600 |
| R-factor | 0.1489 |
| Rwork | 0.147 |
| R-free | 0.18830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qj5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.974 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | BALBES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.940 | 70.940 | 1.630 |
| High resolution limit [Å] | 1.600 | 8.760 | 1.600 |
| Rmerge | 0.106 | 0.059 | 0.836 |
| Rmeas | 0.115 | 0.064 | 0.908 |
| Rpim | 0.045 | 0.023 | 0.350 |
| Total number of observations | 911100 | 6877 | 46704 |
| Number of reflections | 143372 | 968 | 7224 |
| <I/σ(I)> | 10.6 | 28.2 | 2.6 |
| Completeness [%] | 97.0 | 99.8 | 98.6 |
| Redundancy | 6.4 | 7.1 | 6.5 |
| CC(1/2) | 0.997 | 0.995 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.1 | 293 | Sodium Citrate 0.1M pH 6.1; Potassium Sodium Tartrate 0.2M; Ammonium sulfate 1.6M |
