6EQL

Crystal Structure of Human Glycogenin-1 (GYG1) Tyr195pIPhe mutant complexed with manganese and UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	66.170, 81.050, 111.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.550 - 2.380
R-factor	0.228
Rwork	0.225
R-free	0.28070
RMSD bond length	0.006
RMSD bond angle	0.440
Data reduction software	xia2
Data scaling software	Aimless (0.5.1)
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.550	46.550	2.440
High resolution limit [Å]	2.380	10.640	2.380
Rmerge	0.159	0.029	1.731
Rmeas	0.173	0.032	1.876
Rpim	0.068	0.014	0.718
Total number of observations	159048
Number of reflections	24661	331	1799
<I/σ(I)>	9.2
Completeness [%]	99.9	99.1	99.8
Redundancy	6.4	5.2	6.7
CC(1/2)	0.997	0.999	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	22.5% PEG Smear High, 0.2M potassium chloride