6ENP
Atomic resolution structure of human RNase 6 in the presence of phosphate anions in P21 space group.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.734, 38.557, 98.965 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.927 - 1.042 |
R-factor | 0.1227 |
Rwork | 0.121 |
R-free | 0.14490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x09 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.424 |
Data reduction software | XDS (20151001) |
Data scaling software | Aimless (0.5.17) |
Phasing software | PHENIX (1.11.1_2575) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.930 | 1.079 |
High resolution limit [Å] | 1.042 | 1.042 |
Rmerge | 0.032 | 0.506 |
Rmeas | 0.038 | 0.019 |
Rpim | 0.019 | 0.306 |
Number of reflections | 95760 | 4946 |
<I/σ(I)> | 17.08 | 2.4 |
Completeness [%] | 97.2 | 97.13 |
Redundancy | 3.5 | 3.4 |
CC(1/2) | 0.999 | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1 M ammonium phosphate, 100 mM sodium acetate pH 4.5 |