6EN2
Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex with a circular intermediate DNA (CI6b-DNA)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976300 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 79.928, 132.725, 125.033 |
Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
Resolution | 68.471 - 2.670 |
R-factor | 0.1877 |
Rwork | 0.185 |
R-free | 0.24440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6en1 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.471 | 2.550 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmeas | 0.074 | 1.156 |
Number of reflections | 42632 | 2939 |
<I/σ(I)> | 11.85 | |
Completeness [%] | 92.5 | 87 |
Redundancy | 2.6 | 2.4 |
CC(1/2) | 0.998 | 0.444 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1M Na Acetate pH4.6, 30% PEG300 |