6ELC

Crystal Structure of O-linked Glycosylated VSG3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9194
Spacegroup name	I 21 3
Unit cell lengths	129.854, 129.854, 129.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.820 - 1.410
R-factor	0.13193
Rwork	0.130
R-free	0.16921
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.758
Data reduction software	XDS (June 17, 2015)
Data scaling software	XDS (June 17, 2015)
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.820	1.430
High resolution limit [Å]	1.410	1.410
Rpim	0.022	0.725
Number of reflections	68683	2732
<I/σ(I)>	19.1	0.6
Completeness [%]	97.9	73.9
Redundancy	4.1	1.6
CC(1/2)	0.999	0.407

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Hanging drops formed from mixing a 1:1 volume ratio of the protein with an equilibration buffer consisting of 16-22% polyethylene glycol (PEG) molecular weight 3350 Da, 200-325mM NaCl, and 100mM Tris pH 8.2 (tris(hydroxymethyl)aminomethane)