6EL8

Crystal structure of the Forkhead domain of human FOXN1 in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	P 1
Unit cell lengths	38.848, 43.284, 58.317
Unit cell angles	90.05, 95.67, 93.77

Refinement procedure

Resolution	34.000 - 1.610
R-factor	0.1959
Rwork	0.194
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ocn
RMSD bond length	0.015
RMSD bond angle	1.474
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.520	1.650
High resolution limit [Å]	1.610	1.610
Rmerge	0.035	0.395
Rpim	0.028	0.333
Number of reflections	44332	1937
<I/σ(I)>	8.1	1.2
Completeness [%]	90.7	53.4
Redundancy	2.1	1.9
CC(1/2)	0.998	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	297	8% Peg 4000, 0.1M Acetate pH4.5