6EJ1

Crystal structure of KDM5B in complex with KDOPZ48a.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-25
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92819
Spacegroup name	P 65 2 2
Unit cell lengths	143.210, 143.210, 153.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	71.605 - 2.070
R-factor	0.1957
Rwork	0.195
R-free	0.21750
RMSD bond length	0.009
RMSD bond angle	0.977
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.610	2.120
High resolution limit [Å]	2.070	2.070
Rmerge	0.131	2.249
Number of reflections	57111	4148
<I/σ(I)>	17.2	1.5
Completeness [%]	100.0	100
Redundancy	19.8	20.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1M HEPES pH 7.5, 0.8M Pottassium Phosphate-dibasic, 0.8M Sodium Phosphate monobasic