6EIB
Structure of the active GGEEF domain of a diguanylate cyclase from Vibrio cholerae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98141 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.905, 80.267, 71.696 |
| Unit cell angles | 90.00, 97.59, 90.00 |
Refinement procedure
| Resolution | 35.534 - 1.941 |
| R-factor | 0.1958 |
| Rwork | 0.194 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zve |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.247 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.410 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 52783 | 8471 |
| <I/σ(I)> | 7.82 | 1.26 |
| Completeness [%] | 96.3 | 95.9 |
| Redundancy | 2.34 | |
| CC(1/2) | 0.995 | 0.687 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.2 | 277 | 0.5M Ammonium sulfate, 0.1M Sodium citrate |
