6EI4
Crystal Structure of tyrosinase from Bacillus megaterium with B5N inhibitor in the active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.650, 78.100, 81.050 |
| Unit cell angles | 90.00, 104.52, 90.00 |
Refinement procedure
| Resolution | 28.098 - 2.000 |
| Rwork | 0.227 |
| R-free | 0.25050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p6r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.901 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.100 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.128 | 0.484 |
| Number of reflections | 71968 | 375111 |
| <I/σ(I)> | 6.5 | 3.1 |
| Completeness [%] | 98.8 | 98.4 |
| Redundancy | 5.2 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG 8000 0.1M sodium cacodylate |
