6EI1
Crystal structure of the covalent complex between deubiquitinase ZUFSP (ZUP1) and Ubiquitin-PA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 84.230, 84.230, 201.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.448 - 1.732 |
R-factor | 0.1727 |
Rwork | 0.171 |
R-free | 0.20320 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.709 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.450 |
High resolution limit [Å] | 1.730 |
Number of reflections | 44866 |
<I/σ(I)> | 16.6 |
Completeness [%] | 99.0 |
Redundancy | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 277 | 0.2 M sodium malonate pH 5, 20 % PEG 3350 |