6EFD
Hsa Siglec and Unique domains in complex with the sialyl T antigen trisaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.645, 58.140, 75.959 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.168 - 1.850 |
R-factor | 0.1968 |
Rwork | 0.196 |
R-free | 0.21760 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.392 |
Refinement software | PHENIX ((1.14_3211)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 30549 | |
<I/σ(I)> | 31.7 | |
Completeness [%] | 98.8 | |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M SPG pH 10, 25% PEG 3350 |