6EEV

Structure of class II HMG-CoA reductase from Delftia acidovorans with mevalonate bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 3 2 1
Unit cell lengths	100.370, 100.370, 75.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.125 - 1.490
R-factor	0.1366
Rwork	0.135
R-free	0.16460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.821
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.125	1.520
High resolution limit [Å]	1.490	1.490
Number of reflections	70102
<I/σ(I)>	10.5
Completeness [%]	97.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100 mM Bis-Tris, pH 6.5, 600 mM lithium sulfate, 19% PEG3350