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6ECJ

Human cytochrome c G41T

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron siteAustralian Synchrotron
BeamlineMX2
Temperature [K]93
Detector technologyCCD
Collection date2015-11-05
DetectorADSC QUANTUM 315r
Wavelength(s)0.95370
Spacegroup nameP 2 21 21
Unit cell lengths65.060, 76.170, 232.830
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution65.060 - 2.700
R-factor0.2166
Rwork0.215
R-free0.24860
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3nwv
Data reduction softwareMOSFLM
Data scaling softwareAimless (0.3.11)
Phasing softwarePHASER (2.6.0)
Refinement softwarePHENIX (1.13-2998)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]77.6102.830
High resolution limit [Å]2.7002.700
Rmerge0.2130.764
Rmeas0.2290.823
Rpim0.0850.303
Total number of observations233952
Number of reflections327534277
<I/σ(I)>7.9
Completeness [%]100.0100
Redundancy7.17.3
CC(1/2)0.9900.852
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.5291Hanging drops of 1 microL of 30.1 mg/mL of reduced protein and 1 microL reservoir buffer were allowed to equilibrate above the reservoir buffer (30% (w/v) polyethylene glycol 5000, 60 mM lithiumsulfate, 50 mM Tris-HCl pH 8.5). The protein solution was 26 mM sodium phosphate, 37 mM sodium chloride, 14 mM sodium dithionite (pH 7.6).

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