6ECJ
Human cytochrome c G41T
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-11-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 65.060, 76.170, 232.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.060 - 2.700 |
R-factor | 0.2166 |
Rwork | 0.215 |
R-free | 0.24860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nwv |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.610 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.213 | 0.764 |
Rmeas | 0.229 | 0.823 |
Rpim | 0.085 | 0.303 |
Total number of observations | 233952 | |
Number of reflections | 32753 | 4277 |
<I/σ(I)> | 7.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 7.3 |
CC(1/2) | 0.990 | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Hanging drops of 1 microL of 30.1 mg/mL of reduced protein and 1 microL reservoir buffer were allowed to equilibrate above the reservoir buffer (30% (w/v) polyethylene glycol 5000, 60 mM lithiumsulfate, 50 mM Tris-HCl pH 8.5). The protein solution was 26 mM sodium phosphate, 37 mM sodium chloride, 14 mM sodium dithionite (pH 7.6). |