6EC6

Ruminococcus gnavus Beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-02
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	88.292, 137.916, 153.837
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.498 - 2.850
R-factor	0.1423
Rwork	0.140
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lpg
RMSD bond length	0.015
RMSD bond angle	1.249
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.500	2.960
High resolution limit [Å]	2.850	2.850
Rmerge	0.116
Number of reflections	293029
<I/σ(I)>	15.9
Completeness [%]	99.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	1 M diammonium hydrogen citrate and 0.05 M sodium acetate, pH 4.5