6EB5
Crystal Structure of Human Protocadherin-15 EC2-3 V250N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.184, 31.945, 115.955 |
| Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
| Resolution | 39.470 - 2.600 |
| R-factor | 0.23393 |
| Rwork | 0.232 |
| R-free | 0.27039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uly |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.198 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.194 | 0.327 |
| Number of reflections | 17491 | 777 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 95.0 | 89.2 |
| Redundancy | 4.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 277 | 0.1 M MES pH 5.9, 0.15 M MgCl2, 32% PEG 400 |
