6E93

Crystal Structure of ZBTB38 C-terminal Zinc Fingers 6-9 in complex with methylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	277.15
Detector technology	PIXEL
Collection date	2018-03-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	70.804, 77.270, 103.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.536 - 1.747
R-factor	0.1886
Rwork	0.186
R-free	0.21120
RMSD bond length	0.007
RMSD bond angle	1.052
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.810
High resolution limit [Å]	1.747	1.750
Number of reflections	29108
<I/σ(I)>	4.9
Completeness [%]	99.8
Redundancy	6.7
CC(1/2)	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	277.15	0.25M Ammonium Chloride 0.1 MMES (pH:5.8) 20% PEG 6000