6E7K

Structure of the lipoprotein lipase GPIHBP1 complex that mediates plasma triglyceride hydrolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	125
Detector technology	PIXEL
Collection date	2017-12-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	101.948, 153.206, 95.783
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.410 - 2.800
R-factor	0.19738
Rwork	0.195
R-free	0.23545
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hpl
RMSD bond length	0.010
RMSD bond angle	1.433
Data reduction software	HKL-2000 (v716.1)
Data scaling software	HKL-2000 (v716.1)
Phasing software	PHASER (v2.7.17)
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.800	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	37662
<I/σ(I)>	13.5	1.5
Completeness [%]	99.4	99.7
Redundancy	3.3	3.2
CC(1/2)	0.985	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM magnesium acetate, 20% PEG3350