6E7H

Crystal structure of H5 hemagglutinin mutant Y161A from A/Viet Nam/1203/2004 H5N1 influenza virus in complex with 3'-GcLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	66.555, 231.070, 71.295
Unit cell angles	90.00, 97.04, 90.00

Refinement procedure

Resolution	47.067 - 3.300
R-factor	0.2416
Rwork	0.239
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e7g
RMSD bond length	0.003
RMSD bond angle	0.856
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.300	3.300
Rpim	0.047	0.480
Number of reflections	31184
<I/σ(I)>	11.4
Completeness [%]	97.3
Redundancy	3.5
CC(1/2)	0.920	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	20% (w/v) PEG 3350, 0.1M Na-Acetate, pH 7.0.