6E7G
Crystal structure of H5 hemagglutinin mutant Y161A from A/Viet Nam/1203/2004 H5N1 influenza virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97962 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.214, 231.136, 70.864 |
| Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
| Resolution | 37.814 - 3.094 |
| R-factor | 0.2241 |
| Rwork | 0.222 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fko |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.732 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rpim | 0.070 | 0.800 |
| Number of reflections | 38317 | |
| <I/σ(I)> | 18 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.910 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 20% (w/v) PEG 3350, 0.1M Na-Acetate, pH 7.0. |
