6E76

Structure of Human Transthyretin Asp38Ala/Thr119Met Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	43.330, 85.330, 63.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.510 - 1.600
R-factor	0.1935
Rwork	0.191
R-free	0.21590
RMSD bond length	0.005
RMSD bond angle	0.823
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.510	63.510	1.640
High resolution limit [Å]	1.600	7.140	1.600
Rmerge	0.049	0.038	1.011
Rmeas	0.051	0.040	1.054
Number of reflections	31882	421	2261
<I/σ(I)>	25.84	53.4	2.49
Completeness [%]	99.8	100	97.9
Redundancy	12.865	11.587	12.56
CC(1/2)	0.999	0.999	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	50% (w/v) PEG-400, 0.2M Lithium Sulfate, 0.1M Sodium Acetate