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6E62

Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody 85RF45.1

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsCLSI BEAMLINE 08ID-1
Synchrotron siteCLSI
Beamline08ID-1
Temperature [K]100
Detector technologyPIXEL
Collection date2018-01-30
DetectorDECTRIS PILATUS3 S 6M
Wavelength(s)0.979490
Spacegroup nameP 21 21 21
Unit cell lengths54.510, 74.450, 321.890
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.697 - 2.700
R-factor0.2472
Rwork0.246
R-free0.27570
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.003
RMSD bond angle0.629
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwarePHENIX ((1.13_2998: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.7002.800
High resolution limit [Å]2.7002.700
Number of reflections372283756
<I/σ(I)>14.41.7
Completeness [%]100.0100
Redundancy13.113.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2930.1 M Bis-Tris, pH 5.5, 0.1 M ammonium acetate, 17% (w/v) PEG 10000

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