6E62
Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody 85RF45.1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979490 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.510, 74.450, 321.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.697 - 2.700 |
R-factor | 0.2472 |
Rwork | 0.246 |
R-free | 0.27570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.629 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.700 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 37228 | 3756 |
<I/σ(I)> | 14.4 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.1 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris, pH 5.5, 0.1 M ammonium acetate, 17% (w/v) PEG 10000 |