6E5B
Human Immunoproteasome 20S particle in complex with compound 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99983 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 118.573, 203.142, 161.487 |
Unit cell angles | 90.00, 106.82, 90.00 |
Refinement procedure
Resolution | 154.580 - 2.770 |
R-factor | 0.2482 |
Rwork | 0.248 |
R-free | 0.29150 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.350 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 154.580 | |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 0.082 | 0.451 |
Rmeas | 0.102 | 0.034 |
Number of reflections | 179824 | 653 |
<I/σ(I)> | 10.64 | 37.45 |
Completeness [%] | 97.0 | 96.5 |
Redundancy | 2.8 | 2.8 |
CC(1/2) | 0.995 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 39% (v/v) 2-methyl-2,4-pentanediol (MPD), 0.2M sodium thiocyanate |